Publication: Localization of the polyol pathway in the human kidney
Authors
Zopf, Steffen ; Flämig, Jakob ; Schmid, Heide ; Miosge, Nicolai ; Blaschke, Sabine ; Hahn, Eckhart G. ; Múller, Gerhard Anton ; Grunewald, Rolf W.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Sorbitol plays an important role in the
osmotic regulation of the mammalian kidney. Sorbitol
synthesis is regulated by the enzyme aldose reductase
(AR) and its degradation to fructose is catalyzed by the
enzyme sorbitol dehydrogenase (SDH). Various data
exist on the polyol pathway on the rat kidney, but little is
known about the distribution of the polyol pathway
enzymes in the human kidney. Determination of enzyme
activities and a semiquantitative determination of mRNA
expression, immunohistochemistry and in-situ
hybridisation in healthy human kidney tissue was carried
out. The enzyme activity of AR showed a fourfold
increase from cortex to papilla, while SDH-activity
dropped from cortex to papilla by a factor of four.
Corresponding data was obtained at the mRNA level
from the semiquantitative polymerase chain reaction
(PCR). Additional differentiation at the cellular level
reveals both enzymes in cells of the proximal and distal
tubules, thick ascending loop, thin loop and collecting
duct. Studies of enzyme activity and expression by
immunohistochemistry, PCR and in-situ hybridization
presented corresponding results with respect to the
localization of the enzymes, which match the experimental data obtained from rats very well. Thus,
the established rat model might well represent the
situation in the human kidney, too.
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