Publication: Safety of isotretinoin treatment as measured by liver parameters
Authors
Thomazini, B.F. ; Lamas, C.A. ; Dolder, M.A.H.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-18-075
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info:eu-repo/semantics/article
Description
Abstract
Isotretinoin is an analogue of vitamin A and
by suppressing the sebaceous glands it is often
prescribed in cases of severe acne treatment. The
treatment for the average patient is carried out during
two to ten months. This study was designed to
investigate liver structure, hepatic enzyme levels and the
stress oxidative parameter after isotretinoin treatment
during a similar period and using the dosages of 1 mg/kg
and another one of 10 mg/kg in young male Wistar rats.
We have analyzed the blood serum biochemical levels to
determine hepatic function and lipid peroxidation,
hepatic tissue levels of hepatic enzymes, histology and
ultrastructure. The groups receiving 1 mg/kg were not
altered after treatment. Their ultrastructure showed a
metabolically more active organ after treatment with 10
mg/kg, in which there was an increase in the area
occupied by mitochondria and rough reticulum in
electron transmission images. The group that received 10
mg/kg also showed increased alkaline phosphatase,
decreased high density lipoprotein and low density
lipoprotein. The changes observed with the 10 mg/kg
dose were not conclusive for liver damage, because of
the lack of histological structural modifications and the
few biochemical alterations. The 1 mg/kg dose showed a
liver responding to some stimuli but without profound
alterations. So, we confirm that the proposed protocol
with 1 mg/kg or 10 mg/kg isotretinoin did not cause
important biochemical and histological disfunctions for
male Wistar rat livers.
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