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Increased expression of 5-lipoxygenase is common in clear cell renal cell carcinoma

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dc.contributor.authorFaronato, M.es
dc.contributor.authorMuzzonigro, G.es
dc.contributor.authorMilanese, G.
dc.contributor.authorMenna, C.
dc.contributor.authorBonfigli, A.R.
dc.contributor.authorCatalano, A.
dc.contributor.authorProcopio, A.
dc.date.accessioned2012-05-21T12:11:07Z
dc.date.available2012-05-21T12:11:07Z
dc.date.issued2007
dc.description.abstractThe clinical behaviour of Clear Cell Renal Cell Carcinoma (CC-RCC) is often unpredictable. To fully understand the signaling pathways involved in CCRCC development, we examined whether the 5- Lipoxygenase (5-LO), which catalyzes the biosynthesis of proinflammatory leukotrienes, is involved in renal tumorigenesis. By analyzing 46 snap-frozen primary renal cell carcinomas and their corresponding normal renal cortex biopsies, 5-LO protein levels were found to be significantly increased in the majority of CC-RCCs (P<0.001). Quantitative 5-LO mRNA expression analysis revealed up to 3-fold increased expression in the tumor tissues. There was no association between 5-LO and gender, grade or vein invasion. In contrast, increased 5-LO protein and mRNA correlated with large tumor size (>4 cm) and age of patients (P<0.001). 5-LO was frequently overexpressed in von Hippel-Lindau protein (pVHL)–reduced tumors and in Vascular Endothelial Growth Factor (VEGF)-positive tumors, which represent two frequent alterations in CC-RCC. Cell culture experiments demonstrated that VEGF expression was strongly inducible by 5-LO metabolites in RCC cell lines. The loss of pVHL expression led to high basal 5- LO and VEGF expression, which were markedly reduced by transfection with 5-LO small interfering RNA (siRNA). These results suggest that 5-LO upregulation is an important step in renal cancer progression.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/27642
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectRenal cell carcinomaes
dc.subjectTumor progressiones
dc.subject.other616.6 - Patología del sistema genitourinarioes
dc.titleIncreased expression of 5-lipoxygenase is common in clear cell renal cell carcinomaes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.22, nº10 (2007)