Publication: Immunocytochemical developmental patterns of the thoracolumbar sympathetic chain in
the chick and a comparison with its adrenal counterpart
Authors
Sánchez-Montesinos, I. ; Mérida-Velasco, J.R. ; Hita-Contreras, F. ; Espín Ferra, J. ; Rodríguez-Vázquez, J.F. ; De La Cuadra, C. ; Pasini, B. ; Mérida-Velasco, J.A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The immunocytochemical development of
the thoracolumbar sympathetic ganglion and its adrenal
counterpart was studied in the chick from days 3.5 to 12
of incubation, using antibodies to 17 separate antigens,
including antibodies to pan-neuroendocrine markers,
catecholamine-synthesizing and proprotein-processing
enzymes, and neuropeptides. Some of the antigens
studied (Go protein-a subunit, thyrosine hydroxylase,
and galanin) were strongly expressed from the first days
of development, whereas others (chromogranin-A,
chromogranin-B, 7B2 protein, and somatostatin) showed
a diverse immunoreactive expression at different stages.
Three different patterns were found in the development
of both adrenal medulla and thoracolumbar sympathetic
ganglion. In the first (chromogranin-A and B, Go
protein-a subunit, tyrosine hydroxylase, HNK-1, and
galanin), virtually all medullary and thoracolumbar
sympathetic ganglion cells were strongly immunostained
from day 4 onward. Except for HNK-1, chromogranin-A
and B, there was a steady increase in immunoreactive
cells for all the remaining antigens up to day 12. In the
second (7B2 protein, proprotein convertase 2, and
secretogranin II), full antigenic expression was reached
in medullary and thoracolumbar sympathetic ganglion cells by day 10. In the third pattern (proprotein
convertase 3, somatostatin, dopamine-ß-hydroxylase,
neuron-specific enolase, vasoactive intestinal
polypeptide, and met-enkephalin), differences in
immunoreactivity were observed between the medullary
and thoracolumbar sympathetic ganglion cells.
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