Publication: Protein markers in the microcyst of the posteroventral cochlear nucleus of the gerbil
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Date
2008
Authors
Yu, S. M. ; Lin, K. H. ; Lai, Y.L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Microcysts are most evident in the
posteroventral and anteroventral cochlear nuclei (PVCN
and AVCN) of the Mongolian gerbil. The origin and
contents of the microcyst are not elucidated at present.
The present study investigated the possible inclusions in
the microcyst by employing immunocytochemical
labeling to localize the existence of various protein
markers. Thirty and 100 μm thick sections were used to
substitute and reconstruct between 6 and 20 paraffin
serial sections, respectively. In 30-μm-thick slice
sections, immunoreactivity of glial fibrillary acidic
protein (GFAP-IR), mitochondria inner membrane
(MCA-In-IR), S-100 (S-100-IR), serotonin (5-HT-IR),
myelin proteolipid protein (PLP-IR) and substance P
(SP-IR) abutted on the perimeter of the microcyst. The
immunolabeled SP-positive cells were adjacent to the
evagination of the microcyst. In 100-μm-thick slice
sections, immunoreactivity of nitric oxide synthase
(NOS-IR) and somatostatin (SOM-IR) mainly
precipitated as flocculent structures in the small to
medium-sized microcysts. 5-HT-IR also precipitated as
an elongated flocculent stalk adjacent to the large microcyst or randomly distributed in the neuropil. The
findings suggest that GFAP, MCA-In, S-100, 5-HT, PLP,
SP, NOS and SOM may be involved in modulating the
physiological functions and maintaining microenvironmental
homeostasis of the microcyst in the
cochlear nucleus of the gerbil.
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