Publication: lntravesicular Fas localization in epithelial cells of castrated rat prostate glands
Authors
Serizawa, Y. ; Ueda, H. ; Baba, T. ; Ueno, A. ; Takeda, M. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Androgenic steroids regulate the
development and size of mammalian prostate epithelial
cells. To evaluate the relationship between Fas-Fas
ligand system and apoptosis in prostate epithelial cells of
the castrated rats, we have examined immunocytochemical
localization of Fas antigen in the castrated rat
prostate glands at a series of different times. We used a
rabbit polyclonal anti-Fas antibody with a streptavidinbiotin
method and confocal laser scanning method or an
immunogold method. Fas immunolocalization was
exarnined in ventral lobes of prostate glands taken from
intact or castrated adult male Wistar rats on day 1,2,3,4
and 5 by light or electron microscopy. At a light
microscopic level, the castrated prostate epithelial cells
showed mostly Fas immunolocalization in their apical
parts of cytoplasm on day 2 after the castration. In
addition, their extent of the Fas expression was
expanded throughout the cytoplasm in proportion to the
androgen ablation periods, and later the Fas expression
was detected at luminar or basolateral sides of the
epithelial cells. Both immunogold labeling with ultrathin
sections and immunoperoxidase technique with cryostat
sections demonstrated that Fas was localized mainly in
secretory granules of the castrated prostate epithelial
cells and some parts of their cell membranes at later
stages. Our immunocytochemical findings showed that
Fas expression was time-dependently induced in most of
the prostatic epithelial cells after castration of rats. The
rate of Fas-expressing epithelial cells was too high and inconsistent with the previously reported rate of
TUNEL-positive ones. The membrane-associated Fas
may have little effect on the apoptosis in the present
case, bacause a lot of soluble Fas was secreted from the
prostatic epithelial cells. A further study is needed to
clarify some significance of the secretory Fas in the
prostatic epithelium after the rat castration.
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