Publication: Do adhesion molecules importantly regulate leukocyte kinetics within intraacinar microvessels of the lung.
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Date
1998
Authors
Yamaguchi, K. ; Nishio, K. ; Aoki, T. ; Suzuki, Y. ; Sato, N. ; Takeshita, K. ; Kudo, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Precise assessment of blood cell kinetics in
the pulmonary microcirculation is extremely difficult
because pulmonary microvascular architecture contains
arterioles, venules and capillaries in an exceedingly
intricate and densely convoluted fashion. Conventional
epiluminescence microscopy may not be suitable for
investigation of blood cell kinetics in the pulmonary
microcirculation, in which arterioles, venules and
capillary networks are not located in the same plane. To
overcome these impediments, we recently developed a
real-time confocal laser luminescence microscope with a
high-speed analysis component having the capacity to
yield confocal-images of rapidly moving cells at a
rate of 1,000 frameslsec and at sufficiently high
magnification. In the current review, we will first
introduce the details of our newly developed observation
system constructed with a view to estimation of blood
cell dynamics in the intraacinar microcirculation of the
lung. Applying this novel method to isolated perfused rat
lungs, we will secondly address the issue of whether or
not leukocyte-endothelium interactions in the pulmonary
microcirculation qualitatively differ from those serving
in the systemic microcirculation. We will particularly
shed light on possible roles of endothelial ICAM-1,
endothelial P-selectin and leukocyte L-selectin in
distorting leukocyte kinetics in the intraacinar microvessels
under a variety of diseased conditions, including
prolonged exposure to a hyperoxic environment
inducing a significant upregulation of ICAM-1 as well as
P-selectin on the pulmonary microvascular endothelium,
and stimulation of leukocytes by an IL-8 analog causing
downregulation of leukocyte L-selectin but inverse
upregulation of CD18-related integrins.
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