Publication: Down-regulation of TSG101 by small
interfering RNA inhibits the proliferation of breast
cancer cells through the MAPK/ERK signal pathway
Authors
Zhang, Y. ; Song, M. ; Cui, Z.S. ; Li, C.Y. ; Xue, X.X. ; Yu, M. ; Lu, Y. ; Zhang, S.Y. ; Wang, E.H. ; Wen, Y.Y.
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We designed to investigate the effects of
down-regulating the tumor susceptibility gene 101
(TSG101) on the proliferation and apoptosis of the
human breast cancer MCF-7 cell line, and the role of the
MAPK/ERK signal pathway in this process. The siRNA
against TSG101 was transfected into the breast cancer
MCF-7 cell line using Lipofectamine 2000. After
TSG101 knockdown, the proliferation of MCF-7 cells
was measured by the MTT assay. The cell cycle
distribution and apoptosis were examined by using flow
cytometry while cell migration was measured using a
transwell assay. The protein level of p-ERK was further
assessed by immunofluorescence and western blotting.
Our results are as following, the MCF-7 cells transfected
with TSG101 siRNA proliferated significantly slower
and exhibited significantly increased rates of apoptosis
compared to the control cells. In the TSG101 siRNA
transfected cells, the percentage of cells in the G0/G1 and
S phase of the cell cycle was significantly higher and
lower, respectively, compared to the control cells.
Moreover, the migration ability of TSG101 siRNA
transfected cells was lower than the control groups.
Lastly, the level of p-ERK protein in TSG101 siRNA
transfected cells was significantly decreased compared
with the control cells. In conclusion, TSG101
knockdown in breast cancer cells induces apoptosis and
inhibits proliferation. The TSG101 depleted cells are
arrested at the G1/S transition of the cell cycle. The
migration of breast cancer cells is also impaired by
TSG101 siRNA. TSG101 may play a biological role
through modulation of the MAPK/ERK signaling
pathway in breast cancer.
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