Publication: Phase-contrast microscopy of the primate retina
Authors
Fix, James D.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The study of hematoxylin and eosin stained
thick sections (15 pm) of the primate retina with the phasecontrast
microscope provided a means for the selective
demonstration of many cellular structures that could not
be resolved with the same degree of detail which was
possible when bright-field microscopy was used, or when
phase-contrast microscopy was employed to examine
unstained material. The H & E-stain greatly enhanced the
phase-contrast image, so that cytoplasmic structure, fiber
trajectories, and gross synaptic detail of the retina could
be demonstrated to better advantage.
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