Publication: Occurrence of two NOS isoforms in the developing gut of sea bass Dicentrarchus labrax (L.)
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Date
2007
Authors
Pederzoli, A. ; Conte, A. ; Tagliazucchi, D. ; Gambarelli, A. ; Mola, L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In this work we have examined the
appearance and distribution of nitric oxide synthase
(NOS), with histochemical, immunohistochemical and
biochemical methods, during development of the sea
bass (Dicentrarchus labrax) gut.
The data showed that both the calcium-calmodulin
dependent neuronal isoform (nNOS) and calciumindependent
inducible isoform (iNOS) are present in the
larval gut of sea bass. The nNOS-immunoreactivity was
present in the epithelial cells and enteric nerve cells of
gut both in the 8-day-old specimens and in the 24-dayold-
larvae. In the adult nNOS-immunoreactivity
disappeared from epithelial cells, remaining in the wall
intramural neurons and fibers. The iNOSimmunoreactivity
was present in the epithelial cells of
24-day-old-larvae and was not detectable in the adult
gut. Western blot analysis and determination of NOS
activity also demonstrated the presence of the two NOS
isoforms, nNOS and iNOS, in the gut of 24-day-old
specimens. The presumably different roles played by the
two isoforms of enzyme are discussed. The presence of
nNOS isoform in the gut enteric neurons of the same
larval stages of D. labrax in which we previously
demonstrated the presence of substance P and
Vasoactive Intestinal Polypeptide (VIP), may suggest
that all these three components of the motility control system are already present in the larval phase. Nitric
oxide (NO) may be also involved in the early immune
response. The present results on the occurrence of iNOS
isoform in epithelial gut cells of the same regions in
which the gut-associated lymphoid tissue (GALT) will
differentiate, may suggest for NO a role in early defence
mechanisms, before the establishment of immune
responses in GALT. Finally, the developmental and
regional differences in nNOS and iNOS expession also
suggest a regulatory role in development and
differentiation of the sea bass gut.
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