Publication: Stereological study of splenic tissue com partments in FK506-treated rats
Authors
Milicevic, N.M. ; Milicevic, Z.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Model-based stereology was used to study
the changes of splenic structure and proportions of
splenic tissue compartments in FK506-treated male
Wistar rats (0.1 mg/kg three times a week for 14 days).
The point-counting method was utilized to study the
volume densities of the following tissue compartments:
red pulp; white pulp (this compartment was divided in
two subcompartments: follicles and periarteriolar
lymphocyte sheath); marginal zone; aed connective
tissue. The following stereological parameters of
lymphoid follicles were also determined: areal numerical
density (the number of follicles per mm2 of tissue
section); the numerical density (number of follicles per
mm3 of tissue); and the mean follicle diameter. After
FK506 treatment a significant reduction of volume
density of white pulp was observed, which was due to
the very prominent decline in the amount of periarteriolar
lymphocyte sheath, whereby the cellular
density of this tissue compartment was decreased. In the
marginal zone of FK506-treated spleens the presence of
numerous, large, densely packed lymphoblastic cells of
very uniform appearance was registered. These
observations are in very good keeping with the changes
of splenic morphology registered in cyclosporin Atreated
rats, which further documents the parallelism in
actions between these imrnunosuppressive agents.
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