Publication: lmmunohistochemical detection of metallothionein in liver, duodenum and kidney after dietary copper-overload in rats
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Date
1998
Authors
Mullins, J.E. ; Fuentealba, I.C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Metallothionein (MT) has been used in
immunohistochemical techniques to indicate presence
and distribution of heavy metals within biological
tissues. This study describes a comparison of the pattern
of MT-immunostaining in the liver, duodenum and
kidney during dietary copper overload in rats. Sixteen
male 10-week-old Wistar rats were randomly allocated
into groups of four. Two groups were fed a pelleted diet
containing 1,500 mgíkg copper and two control groups
received a rodent diet containing 10 mgkg copper. After
6 weeks samples of liver, kidney and duodenum were
collected for immunohistochemistry and histology. An
indirect immunoperoxidase technique, using monoclonal
antibody E9 against horse MT and polyclonal sera
against rabbit MT, was employed. Copper-loaded rats
had marked MT-immunoreactivity within the nucleus
and cytoplasm of many periportal hepatocytes, renal
proximal convoluted tubule epithelial cells, intestinal
columnar epithelial cells and Paneth cells. Immunohistochemical
staining was similar using either mouse
anti-MT polyclonal serum, or monoclonal antibody E9.
Hepatocytes surrounding inflammatory foci were
positive for MT, supporting the proposed role of this
protein in free radical scavenging. The presence of MTexcretion o€ copper-metallothionein (Cu-MT) in copperloaded
rats. Paneth cells were easily detected using MTimmunostaining.
MT may play a part in absorption of
copper from intestinal contents and possible storage as
CU-MT in Paneth cells. The function of Paneth cells
remains unknown but the presence of marked MTimmunoreactivity
in these cells, observed in copperloaded
rats, suggests their involvement in homeostasis
and metabolism o€ copper.
in the kidney appears to be associated with renal
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