Publication: Sinusoidal 50 Hz magnetic fields do not affect structural morphology and proliferation of human cells in vitro
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Date
2001
Authors
Supino, R. ; Bottone, M.G. ; Pellicciari, C. ; Caserini, C. ; Bottiroli, G. ; Belleri, M. ; Veicsteinas, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The effects of electromagnetic fields on
severa1 processes related to cell physiology and
proliferation are currently being investigated. Although
the results are still not conclusive and even conflicting,
there seems to be a fairly good agreement on the early
effects of electromagnetic fields on the generation of
free radicals and on Ca++-intracellular concentration and
transport. To evaluate the long-lasting consequences of
these precocious events, we examined the effects of
short- and long-term magnetic field exposure on
structural organization (cytokeratin or actin detection),
proliferation (bromodeoxyuridine incorporation and
propidium iodide staining), colony forming ability and
viability (trypan blue exclusion test) of highly
proliferating MCF-7 cells (from human breast
carcinoma) and on slowly proliferating normal human
fibroblasts (from healthy donors). Cells were exposed to
either 20 or 500 pT sinusoidally oscillating (50Hz)
magnetic fields for different lengths of time (1 to 4
days). Short (1 day)- and long (4 days)-time exposure to
the two intensities did not affect MCF-7 growth and
viability, colony number and size, or cellular distribution
along the cell cycle; neither were the cell morphology
and the intracellular distribution and amount of
cytokeratin modified. Similarly, no modifications in the
actin distribution and proliferative potential were
observed in normal human fibroblasts. These findings
suggest that under our experimental conditions,
continuous exposure to magnetic fields does not result in
any appreciable effect in both normal and tumor cells in
vitro.
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