Publication: Differential tissue expression of enhanced
green fluorescent protein in ‘Green mice’
Authors
Ma, De-Fu ; Tezuka, Hideo ; Kondo, Tetsuo ; Sudo, Katsuko ; Niu, Dong-Feng ; Nakazawa, Tadao ; Kawasaki, Tomonori ; Yamane, Tetsu ; Nakamura, Nobuki ; Katoh, Ryohei
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In order to clarify tissue expression of
enhanced green fluorescent protein (EGFP) in ‘green
mice’ from a transgenic line having an EGFP cDNA
under the control of a chicken beta-actin promoter and
cytomegalovirus enhancer, we studied the expression of
EGFP in various organs and tissues from these ‘green
mice’ by immunohistochemistry with anti- EGFP
antibody in conjunction with direct observation for
EGFP fluorescence using confocal laser scanning
microscopy.
On immunohistochemical examination and on direct
observation by confocal laser scanning microscopy, the
level of EGFP expression varied among organs and
tissues. EGFP expression was diffusely and strongly
observed in the skin, pituitary, thyroid gland, parathyroid
gland, heart, gall bladder, pancreas, adrenals and urinary
bladder. There was only sporadic and weak expression
of EGFP in the epithelium of the trachea, bronchus of
the lung, stratified squamous epithelium and gastric
glands of the stomach, hepatic bile ducts of the liver,
glomeruli and renal tubules of the kidney and endometrial
glands of the uterus. Furthermore, EGFP was
only demonstrated within the goblet and paneth cells in
the colon and small intestine, the tall columnar cells in
the ductus epididymis, and the leydig cells in the testis.
In conclusion, our results show that EGFP is
differentially expressed in organs and tissues of ‘green
mice’, which indicates that ‘green mice’ may prove
useful for research involving transplantation and tissue
clonality.
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