Publication: MiR-19b-3p promotes tumor progression of non-small cell lung cancer via downregulating HOXA9 and predicts poor prognosis in patients
Authors
Li, Zu Lei ; Li, Dong ; Yin, Guo Qiang
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-448
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info:eu-repo/semantics/article
Description
Abstract
MiR-19b-3p has been reported in several
types of human cancer. Nevertheless, the expression
profile and biological functions of miR-19b-3p remain
unclear in non-small cell lung cancer (NSCLC). The
expression level of miR-19b-3p was evaluated in
NSCLC tissues and cell lines using qRT-PCR. Survival
analysis was performed using Kaplan-Meier curves,
while the prognostic significance of miR-19b-3p was
analyzed using Cox regression analysis in 80 NSCLC
patients. The effects of miR-19b-3p on cell proliferation
and invasion capacities were analyzed using CCK-8,
crystal violet, and transwell assays. Target genes of miR19b-3p were assessed using luciferase reporter assay,
qRT-PCR, Western blot and rescue experiments. MiR19b-3p was found to be upregulated in human NSCLC
tissues and cell lines. The expression of miR-19b-3p was
observed to be closely associated with TNM stage and
metastasis. High expression of miR-19b-3p was found to
be capable of predicting poor clinical prognosis in
NSCLC patients. Whilst overexpression of miR-19b-3p
was demonstrated to promote the proliferation and
invasion of NSCLC cells, knockdown of miR-19b-3p
showed an opposite inhibitory effect. Bioinformatics
analysis and luciferase reporter assays confirmed that
HOXA9 is a direct target of miR-19b-3p. Functional
assays demonstrated that NSCLC cell proliferation and
invasion were promoted by miR-19b-3p via negative
regulation of HOXA9. Finally, overexpression of
HOXA9 was shown to partially reverse the tumor
promoting effect of miR-19b-3p. This study indicates
that miR-19b-3p is a crucial prognostic biomarker of
NSCLC, and that targeting of the miR-19b-3p/HOXA9
axis may be a promising strategy in NSCLC therapy.
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Citation
Histology and Histopathology Vol. 37, nĀŗ8 (2022)
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