Publication: Ontogeny of reactivity to endothelial cell markers during development of the embryonic and fetal rat lung
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Date
1992
Authors
Han, R.N.N. ; Tanswell, A.K. ; Post, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The reactivity of endothelial cells to putative
endothelial cell-specific markers varies with species,with
vessel size and with the organ studied. To determine
their value in studies of fetal rat lung, and whether organ
immaturity would also influence reactivity, we studied
endothelial cell immunoreactivity to antibodies against
Factor VIIIIvon Willebrand factor (VIII/vWF), and
binding reactivity to Bandeiraea (Griffonia) simplicifolia 1
lectin (BSL 1) during rat fetal lung development. Using
an indirect immunofluorescent technique to detect Factor
VIIYvon Willebrand factor (VIIUvWF), endothelial cells
lining the aortic arches were identified as early as day 11
of gestation (term= 22 days), prior to lung development.
Immunoreactivity to VIIIIvWF was subsequently
localized to intrapulmonary endothelial cells and was not
dependent on vessel size. In contrast, binding reactivity
of FITC-conjugated BSL 1 was observed to both
endothelial cells and to the basement membrane of
developing airways, thus limiting its value as endothelial
cell marker. During very early lung development solitary
angioblasts could not be identified by reactivity to either
VIIYvWF antibodies or to BSL 1, and neither marker
appears to be of value for studies of early angiogenic
events.
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