Publication: Altered expression of urokinase-type plasminogen activator and plasminogen activator inhibitor in high-risk soft tissue sarcomas
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Date
2007
Authors
Benassi, M.S. ; Ponticelli, F. ; Azzoni, E. ; Gamberi, G. ; Pazzaglia, L. ; Chiechi, A. ; Conti, A. ; Spessotto, P. ; Scapolan, M. ; Pignotti, E. ; Bacchini, P. ; Picci, P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In recent years, classification of soft-tissue
sarcomas (STS) has improved with cytogenetic analyses,
but their clinical behavior is still not easily predictable.
The aim of this study was to detect alterations in the
urokinase-type plasminogen system, involved in tumor
growth and invasion, by comparing mRNA levels of its
components with those of paired normal tissues, and
relating them with patient clinical course. Real-time
PCR was performed on human STS cell lines and tissues
from highly malignant STS, including leiomyosarcomas
and malignant fibrous histiocytomas, to evaluate the
expression of urokinase-type plasminogen activator
(uPA), uPA receptor (uPAR) and plasminogen activator
inhibitor-1 (PAI-1). Immunohistochemistry of gene
products was also performed.
Median mRNA values of all genes studied were
higher in tumors than in paired normal tissues. In
agreement with data on STS cell lines, significant upregulation
for uPA and PAI-1 genes compared to
reference values was seen. Moreover, different levels of
expression were related to histotype and metastatic
phenotype. There was accordance between uPA mRNA
and protein expression, while immunodetection of PAI-1
product was weak and scattered.
In recent years, classification of soft-tissue
sarcomas (STS) has improved with cytogenetic analyses,
but their clinical behavior is still not easily predictable.
The aim of this study was to detect alterations in the
urokinase-type plasminogen system, involved in tumor
growth and invasion, by comparing mRNA levels of its
components with those of paired normal tissues, and
relating them with patient clinical course. Real-time
PCR was performed on human STS cell lines and tissues
from highly malignant STS, including leiomyosarcomas
and malignant fibrous histiocytomas, to evaluate the
expression of urokinase-type plasminogen activator
(uPA), uPA receptor (uPAR) and plasminogen activator
inhibitor-1 (PAI-1). Immunohistochemistry of gene
products was also performed.
Median mRNA values of all genes studied were
higher in tumors than in paired normal tissues. In
agreement with data on STS cell lines, significant upregulation
for uPA and PAI-1 genes compared to
reference values was seen. Moreover, different levels of
expression were related to histotype and metastatic
phenotype. There was accordance between uPA mRNA
and protein expression, while immunodetection of PAI-1
product was weak and scattered.
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