Publication: Exercise preconditioning reduces acute ischemic renal injury in Hsp70.1 knockout mouse
Authors
Lee, Jin ; Park, Sok ; Kim, Won Kyu
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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Description
Abstract
Backgroun/aims: Heat shock protein 70
(Hsp70) is an anti-apoptotic protein that has a protective
effect in renal ischemic injury. Exercise up-regulates
Hsp family proteins (Hsps), antioxidants and antiapoptotic
proteins. We hypothesized that exercise as
preconditioning could attenuate acute renal dysfunction
and apoptosis resulting from renal ischemic injury under
the Hsp70 deficient circumstance and could contribute to
the prevention of renal injury induced by ischemia.
Method: To investigate the effect of exercise
preconditioning on protecting the kidney from ischemia
in Hsp70 deficiency, we measured apoptosis-related
factors and Hsps. Hsp70.1 KO and wild-type mice were
divided into sham control (Sham), exercise
preconditioning (Ex), renal ischemia-after-exercise
(Ex+IR), and ischemia (IR) groups. Where appropriate a
treadmill exercise was performed at 20 m/min, 60 min
per day on a 0% gradient for 7 days, and renal ischemia
was induced by clamping the renal pedicle for 25 min.
To characterize the effects of exercise on oxidative
stress- and apoptosis-related factors, and Hsp27 and 70
expressions, we performed immunohistochemistry,
western blotting and TUNEL assay, and also measured
level of serum creatinine.
Results: Serum creatinine concentration and 4-HNE
expression were raised in the IR group, as were caspases
3, 7 and 9, while Cu- and Mn-SOD levels were reduced,
as were those of anti-apoptotic proteins Bcl-XL, Bcl-2
and Hsp27. All these effects were largely reversed by the
exercise preconditioning, as was the decrease in
apoptotic cells observed after exercise preconditioning.
Conclusion: Exercise preconditioning has a
beneficial effect in inhibiting oxidative stress and
apoptotic cell death in the kidney resulting from
ischemic injury even under Hsp70 deficiency.
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Citation
Histology and Histopathology, vol. 28, nº 9 (2013)
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