Publication: Immunohistochemical localization of phosphatidylcholine in rat mandibular condylar
surface and lower joint cavity by cryotechniques
Authors
Zea-Aragón, Z. ; Terada, N. ; Ohtsuki, K. ; Ohnishi, M. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The immunolocalization of phospholipids
has not yet been clearly demonstrated in
temporomandibular joints (TMJs). We have examined
the distribution of one of phospholipids, phosphatidylcholine
(PC), in the rat mandibular condylar surface and
lower joint cavity. Some fresh resected TMJs with their
disks attached were immediately plunged into
isopentane-propane cryogen (-193 °C). Cryostat sections
were cut, mounted on NH3
+-coated slides, and fixed
with paraformaldehyde (PF). Cryosections were first
immunostained with anti-mouse PC antibody (JE-1).
Subsequently, they were labeled with immunogold
particles following silver enhancing for light
microscopic analyses. Some cryosections were subjected
to double immunofluoresecence labeling with antifibronectin
antibody or hyaluronic acid-binding protein
in combination with the anti-PC antibody. As an
immunocontrol, other cryosections were pretreated with
phospholipase A2 before such immunofluorescence
labeling. We have confirmed the presence of PC in the
lower joint cavity of rat TMJs as well as on the
mandibular condylar surface layer, which was
colocalized with hyaluronic acid and fibronectin
respectively. However, by treatment with phospholipase A2, such immunolabeling for PC was clearly decreased,
showing that the PC is a component in the rat in vivo
TMJ. These findings suggest that PC, hyaluronic acid
and fibronectin may interact each other in the TMJ
articular surface areas to play a functional role for
lubrication in TMJ.
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