Publication: Differential expression of proteins related
to smooth muscle cells and myofibroblasts
in human thoracic aortic aneurysm
Authors
Forte, Amalia ; Della Corte, Alessandro ; Grossi, Mario ; Bancone, Ciro ; Maiello, Ciro ; Galderisi, Umberto ; Cipollaro, Marilena
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Objectives: Increasing knowledge is required
for a better comprehension of the etiology of thoracic
aortic aneurysm (TAA). The aim of this study was to
highlight the modulations in vascular cell phenotypes,
including myofibroblasts (MFs), in human TAA
specimens compared to healthy aortas.
Methods: histology, RT-PCR and immunohistochemical
analysis of a panel of molecules, including EDA
Fibronectin (Fn), smoothelin, CD34 and alpha-smooth
muscle actin (alpha-SMA), selected on the basis of their
informative potential as markers of smooth muscle cells
(SMCs) and MF phenotypic modulation, were
performed on all samples.
Results: The media of TAAs was characterized by
the absence of smoothelin, the unaltered expression of
alpha-SMA accompanied by an alteration of its
distribution pattern, and by the activated expression of
the ED-A isoform of Fn.
We found a concentration of round-shaped cells
exclusively in the adventitia and in the perivascular
tissue of TAAs, also rich in vasa vasorum, largely
expressing alpha-SMA, while a sub-population also
expressed ED-A Fn and CD34.
CD34 was expressed by several cells in the intima of
TAAs, together with cells expressing cytoplasmatic EDA
Fn and alpha-SMA in comparison to healthy aortas.
Conclusion: TAA specimens show an altered
expression and localization of SMC and MF
differentiation markers in comparison to healthy aortas,
with possible implications on remodeling.
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