Publication: Stereological study of tissue compartments of the human spleen
Authors
Milicevic, Z. ; Cuschieri, A. ; Xuereb, A. ; Milicevic, N.M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Morphometric reports on animal and human
spleen are very few and no studies have been carried out
using stereological methods to investigate all of the
tissue compartments of the human spleen. Eighteen
samples of spleens, which were either surgically
removed after traumatic injury or during treatment for
early stage carcinoma or gastric ulcer, were investigated.
The point-counting method was used to study the
volume densities of the following tissue compartments:
red pulp, perifollicular zone, white pulp (this tissue
compartment was divided in two subcompartments:
follicles and periarteriolar lymphatic sheath), marginal
zone and connective tissue of trabeculas. The following
stereological parameters of the follicles were
investigated: the number of follicles per mm2 of spleen
section, numerical density, volume density, and the mean
follicular diameter. The identity of spleen tissue
compartments was verified using immunohistochemical
staining for B- and T-lymphocytes. The volume densities
of tissue compartments, as well as stereological
parameters of lymphoid follicles, were similar in both
groups of splenic samples, except for the volume
densities of perifollicular zone and periarteriolar
lymphatic sheath, where a statistically significant
difference was registered.
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