Publication: Histological study of timing and
embryology of notochordal abnormalities
in rat exposed in utero to Doxorubicin
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Date
2002
Authors
Menegola, E. ; Broccia, M.L. ; Di Renzo, F.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Experimental Doxorubicin-exposure in utero
is correlated with foetal oesophageal atresia, tracheooesophageal
fistula, axial alterations. While gastrointestinal
and respiratory defects have been larg e l y
investigated, only sporadic data have been published to
date on notochordal and vertebral defects. The aim of
this work was the study of the genesis of chordal and
vertebral abnormalities in rat embryos and foetuses
exposed to Doxorubicin and the study of their
correlation with oesophageal and tracheal defects.
For this purpose, pregnant rats were i.p. injected
with saline (control) or with 4mg/Kg b.w. Doxorubicin
on days 9.5 and 10.5 of gestation. Embryos and foetuses
were morphologically analysed on days 10.5-15 and 16,
18, 20 of gestation respectively, fixed in formaldehyde
and histologically processed. Slides were routinely
stained with haematoxylin-eosin (11-15 days post coitum
embryos and all foetuses) or specifically stained with
aniline blue for the staining of basal laminae (10.5 days
post coitum embryos). Moreover, some foetuses at term
(20 days post coitum) were processed for bone and
cartilage staining.
The data obtained in the present work confirm the
specificity of Doxorubicin in inducing gastro-intestinal
and tracheal defects, describe the genesis of these
defects step by step, describe the type and the genesis of
notochordal abnormalities and their fate and exclude the
role of Doxorubicin in inducing axial skeletal
malformations.
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