Publication: Expression of annexin AI in conventional renal cell carcinoma (CRCC) correlates with tumour stage, Fuhrman grade, amount of eosinophilic cells and clinical outcome
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Date
2007
Authors
Zimmermann, U. ; Woenckhaus, C. ; Teller, S. ; Venz, S. ; Langheinrich, M. ; Protzel, C. ; Maile, S. ; Junker, H. ; Giebel, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
There is increasing evidence that Annexin AI
(ANX AI) expression is dysregulated in several
carcinomas and tumour cell lines. In order to gain insight
into the putative role of ANX AI in tumorigenesis,
clinical outcome and metastatic potential of conventional
renal cell carcinomas (CRCCs) we investigated the
expression of ANX AI in CRCCs and metastases.
Furthermore, it was elucidated whether ANX AI
overexpression affects migratory potential in Caki-1
cells.
ANX AI immunohistochemistry was performed on
33 samples of CRCCs and 10 metastases. ANX AI
expression was assessed in 12 samples by 2-dimensional
gelelectrophoresis (2-DE), subsequent mass
spectrometry and RT-PCR. Immunohistochemical data
were statistically correlated with pathological
parameters, amount of eosinophilic cells and clinical
outcome. Furthermore, a haptotactic migration assay was
done on Caki-1 cells transfected with ANX AI.
Immunostaining for ANX AI was found in 18
tumours and all metastases investigated. Intensity of
immunohistochemical staining correlated to Fuhrman
grade, amount of eosinophilic cells and clinical outcome.
2-DE and RT-PCR confirmed the presence of ANX AI in
neoplastic tissue. Overexpression of ANX AI did not
significantly influence cell migration.
From these findings ANX AI expression seems to be
related to Fuhrman grade, clinical outcome and
metastatic potential of CRCCs. Thus ANX AI could
serve as a prognostic marker for tumour progression.
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