Publication: Morphological evidence of telocytes in DHEA-induced polycystic ovary syndrome model
Authors
Mehmet Yüncü ; Yurdun Kuyucu ; Esma İşçel
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Publisher
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Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-25-015
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info:eu-repo/semantics/article
Description
Abstract
Polycystic Ovary Syndrome (PCOS) is a
condition causing histopathological alterations in the
ovarian stroma. Telocytes (TCs) are specialized
interstitial/stromal cells present in the connective tissue
of various organs. In this study, we investigated the
presence and spatial organization of TCs in the ovaries
of a rat model of PCOS induced by dehydro
epiandrosterone (DHEA). The ovarian tissues from both
PCOS and control groups were stained using
hematoxylin-eosin (H&E), Bielschowsky's silver stain,
methylene blue, and toluidine blue for light microscopy
analysis, and scanned digitally. Ovaries were marked
with double-labeled immunofluorescence with
CD34/estrogen receptor-α (ER-α) and vimentin/
progesterone receptor-A (PR-A) and evaluated with a
confocal microscope. The ultrastructure and telopodes
(TPs) of TCs were also examined by transmission
electron microscopy. TCs were identified in both groups,
localized within follicular walls, adjacent to follicles, in
stromal regions distant from the follicles, and
perivascular areas. CD34/ER-α and vimentin/PR-A cells
were significantly increased in PCOS. In conclusion,
TCs were preserved in the DHEA-induced PCOS model,
and according to our quantitative analysis, their
ultrastructural features were unaffected by the PCOS
microenvironment. Our findings suggest a potential
association between TCs and the pathophysiology of
PCOS. Further studies are necessary to elucidate the
functional relationship of TCs in the development and
progression of PCOS.
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