Publication: Effect of unilateral nephrectomy on renal function of diabetic rats
Authors
Lopes, G.S. ; Lemos, C.C.S. ; Mandarim-de-Lacerda, Carlos A. ; Bregman, R.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Glomerular alterations of experimental
diabetes mellitus are observed in animals submitted to a
reduction in renal mass, suggesting that some
mechanisms responsible for the progression of renal
disease are common. The aim of this study was to
investigate the effect of nephrectomy on the renal
function and morphology of diabetic rats.
Male Wistar rats were divided into 4 groups: control
(C), n=8; diabetic (DM), n=8; non-diabetic
nephrectomized (Nx), n=8; (DMNx), n=9. DM was
induced by streptozotocin (65mg/Kg), and animals were
treated with insulin. After 12 weeks, the glomerular
filtration rate (GFR), renal plasma flow (RPF) and mean
arterial pressure (MAP) were evaluated in
unanaesthetized animals. Glomerular volume (GV),
glomerular sclerosis index (GSI), mesangial volume
density (Vvmes) and glomerular capillary surface
density (Svcap) were also evaluated.
Results show that kidney weight increased in Nx
groups, being higher in DMNx. GFR was higher in Nx
groups as was RPF, being higher in DMNx. RVR was lower in Nx groups, especially in DMNx. MAP was not
different among the groups. RPF and GFR showed a
high correlation for the DMNx group (r=0.95, p=0.02).
The DMNx group showed a correlation between RVR
and GFR (r=-0.96, p=0.005). The GV increased in Nx
groups, and the GSI was higher in DMNx. Vvmes and
Svcap increased in DMNx group.
In summary, Nx groups developed similar degrees of
glomerular hypertrophy, but only DMNx showed an
increased value for GSI. The present data suggest that
the acceleration of glomerular lesions in DMNx animals
was more closely associated to hemodynamic
adaptations than to glomerular hypertrophy.
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