Publication: Lymphocyte interactions with the extracellular matrix of malignant cells in vítro: A morphological and immunocytochemical study
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Date
1993
Authors
Logothetou-Rella, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The interactions of lymphocytes with the
glycosaminoglycans-protease-membrane extracellular
matrix, produced by mixed cell cultures of normal with
malignant cell clones, were examined. Pre-activated and
activated heterologous peripheral lymphocytes were
used. Co-cultures of activated lymphocytes with al1 cell
types used, formed identical cell nodules. Histology of
cell nodules showed that activated lymphocytes were
cytolytic to pure normal or malignant cell clones. On the
contrary, lymphocytes in nodules with mixed cell clones
(normal with malignant cell clones) or embryonic cells,
underwent degeneration changing the fusiform type
tumor nodule into the adenoid type. The adenoid type
cell nodule consisted of cells with high nuclear to
cytoplasm ratio and mitotic activity. In addition,
leukocyte common antigen was deposited in the
extracellular matrix and on the cell membrane of target
tumor cells. Pre-activated lymphocytes, in mixed cell
cultures, failed to lyse the target tumor cells and
underwent abnormal cell divisions, producing subsets
similar to nuclear vlimata, which remained attached to
the extracellular matrix. The morphological and
immunocytochemical observations of lymphocytes were
discussed and attributed to the presence of the specific
extracellular matrix of glycosaminoglycans-proteasemembranes
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