Publication: Effects of triple therapy with
octreotide, galanin and serotonin on a
human colon cancer cell line implanted in mice:
comparison between different routes of administration
Authors
El-Salhy, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A human colon cancer cell line was
implanted subcutaneously in nude mice. After 7 days,
the animals were divided into four groups. The first
group received an intraperitoneal (i.p.) continuous
infusion by an osmotic pump, the second was given i.p.
bolus injections, the third received continuous
subcutaneous (s.c.) infusion by an osmotic pump and the
fourth group was given bolus s.c. injections. Each group
was divided into 2 subgroups. The first subgroup
received triple treatment with octreotide, galanin, and
serotonin, 40 µg/kg body weight/day of each. The
second subgroup was given sterile saline solution.
Treatment lasted for 14 days. The volume and wet
weight of the tumours in all treated groups tended to
decrease, but was statistically significant only in the
group with continuous i.p. infusion. The number of
viable cells tended to decrease in all the treated groups,
but was not statistically significant. Proliferation index
was significantly reduced in mice given triple therapy
i.p. as bolus injection and as continuous infusion, as
compared with their respective controls. The apoptotic
index increased significantly in mice receiving triple
therapy as continuous i.p. infusion as revealed by both
the TUNEL method and by poly (ADP-ribose)
polymerase (PARP) expression. The number of tumour
blood vessels was significantly reduced in the mice
given triple therapy as continuous i.p. infusion, as
compared with controls. There was no statistical
difference between animals treated by different routes,
regarding proliferation or apoptosis of the cancer cells,
or the number or mean luminal area of tumour blood
vessels. The present investigation showed that regardless
of the route of administration, triple therapy with
octreotide, galanin and serotonin generally reduced the
volumes, weights, viable cells, vascularization and proliferation of the tumours, as well as inducing
apoptosis. Continuous i.p. infusion appears, however, to
be the most effective route of administration.
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