Publication: Delayed remodeling in the early period
of fracture healing in spontaneously diabetic
BB/OK rats depending on the diabetic metabolic state
Authors
Follak, N. ; Klöting, I. ; Wolf, E. ; Merk, H.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Several clinical series, analyzing fracture
healing in patients with insulin-dependent type 1
diabetes (IDDM) demonstrated significant incidence of
delayed union, non-union, and pseudarthrosis. The
purpose of this study was to examine the detailed
histomorphometry and histology of bone formation and
remodeling during fracture healing depending on the
diabetic metabolic state in spontaneously diabetic
BB/O(ttawa)K(arlsburg) rats, a rat strain that represents
a close homology to IDDM in man.
A standardized fracture model was chosen and based
on blood-glucose values at the time of surgery (mg%),
postoperative blood-glucose course (mg%) and
postoperative insulin requirements (IU/kg), 100
spontaneously diabetic BB/OK rats were divided into
groups with well-compensated (n=50, 167±77 mg%;
244±68 mg%; 1.8±1.9 IU/kg) or poorly compensated
(n=50, 380±89 mg%; 415±80 mg%; 6.0±1.0 IU/kg)
metabolic state. Fifty LEW.1A rats served as the
normoglycemic controls (97±15 mg%). Ten animals
from each group were killed 1, 2, 3, 4 and 6 weeks after
fracture and specimens were processed undecalcified for
quantitative histomorphometry and for qualitative light
microscopy.
In terms of bone histomorphometry, within the first
four weeks after fracture, severe mineralization disorders
occurred exclusively in the rats with poorly compensated
diabetic metabolic states with a significantly decrease of
all fluorochrome-based parameters of mineralization,
apposition, formation and timing of mineralization in
comparison to the spontaneously diabetic rats with wellcompensated
metabolic states and to the control rats.
This was confirmed histologically.
Early fracture healing in the spontaneously diabetic
BB/OK rats is delayed exclusively in poorly compensated diabetic metabolic states, and 6 weeks after
fracture, histomorphometrically significant deficits in the
measured and dynamically calculated parameters
remain. This study suggests that strictly controlled
insulin treatment resulting in well-compensated diabetic
metabolic states will ameliorate the impaired early
mineralization and cell differentiation disorders of
IDDM fracture healing.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.