Publication:
Histological approach to Bacillus subtilis colony-biofilm: evolving internal architecture and sporulation dynamics

Simple item page
dc.contributor.authorGómez-Aguado, Fernando
dc.contributor.authorCorcuera, María Teresa
dc.contributor.authorGómez-Lus, María Luisa
dc.contributor.authorde la Parte, María Antonia
dc.contributor.authorRamos, Carmen
dc.contributor.authorGarcía-Rey, César
dc.contributor.authorAlonso, María José
dc.contributor.authorPrieto, José
dc.date.accessioned2018-09-10T17:22:46Z
dc.date.available2018-09-10T17:22:46Z
dc.date.issued2013
dc.description.abstractBacillus subtilis has been used as a classic model to study biofilm formation and sporulation process. Colonies of wild-type strains usually have a complex external morphology, but the details of their internal architecture are still undisclosed. Since bacterial biofilms fulfill the criteria to be considered tissues, the aim of this work was to analyse B. subtilis colony-biofilm internal architecture evolution and sporulation dynamics using histological techniques. Transversal sections of colony-biofilms incubated from 24 hours up to 20 days were stained using histochemical techniques to analyse the internal structure by light and electron microscopy. A morphometric study of the different structural biofilm components was performed by image analysis, and an application to quantify spores was developed. Internal biofilm architecture was characterised by a stratified pattern, which evolved from 3 strata at 24 hours, up to 5 strata at 20 days. At 48 hours, strata at the central area of the biofilm was folded, resulting in elevated structures (vein-like structures) that could reach up to 465 µm in height. Sporulation started at 48 hours, at the top of the vein-like structures, at the interface between the two uppermost strata. At 20 days spores formed a continuous central layer, representing 7.5% of the total biofilm. In summary, our results demonstrate that B. subtilis colony-biofilm has a complex and organized internal architecture, evolving over time, and taking place in different cell subpopulations with different functionalities. Furthermore, in situ spore quantification described in this work could be a good alternative to the classical chamber counting.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.eisbnHistology and Histopathology, vol. 28, nº 10 (2013)es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/60960
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectBacillus subtilises
dc.subjectBiofilmes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleHistological approach to Bacillus subtilis colony-biofilm: evolving internal architecture and sporulation dynamicses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Gomez-Aguado-28-1351-1360-2013.pdf
Size:
12.81 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.39 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.28, nº10 (2013)