Publication: Significant up-regulation of 1-ACBP, B-ACBP and PBR genes in immune cells within the oesophageal malignant tissue and a possible link in carcinogenic angiogenesis
Authors
Dlamini, Zodwa ; Mbele, Mzwandile ; McCabe, Michelle ; Rees, Jasper ; Naicker, Saraladevi ; Mbita, Zukile
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-11-805
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Oesophageal cancer ranks as the sixth most
common malignancy in the world, and recent evidence
has shown that its incidence is increasing. ACBPs (AcylcoA binding proteins) act as intracellular carrier-proteins
for medium to long chain acyl-coA, mediating fatty acid
transport to the mitochondrion for β-oxidation. ACBPs
are also believed to be putative ligands of PBR
(peripheral benzodiazepine receptor), and once they bind
to this receptor they facilitate mitochondrial membrane
permeabilization, presumably favouring apoptosis. The
main aim of the study was to establish the expression
patterns of 1- Acyl-coA binding proteins (1-ACBP), BAcyl-coA binding proteins (B-ACBP), and peripheral
bezodiazepine receptor (PBR) in oesophageal cancer,
and to link their roles with the disease. In situ
hybridization and quantitative real-time PCR methods
were performed to determine localization and the
expression levels of the three genes in oesophageal
cancer. All three genes products illustrated substantial
up-regulation within the malignant tissue sections as
compared to normal oesophageal sections. All three
transcripts localized specifically to mast cells, plasma
cells and lymphocytes in diseased and normal tissue
section. In the diseased tissue, B-ACBP and 1-ACBP
mRNA localized to endothelial cells of blood vessels in
the submucosa. B-ACBP also localized to the nucleus of
squamous epithelial cells. PBR localization was found in
tumour islands of invasive tissue sections. Quantitative
RT-PCR also indicated that the expression levels of PBR
were higher as compared to the ACBP genes expression
in tumours. These results show that 1-ACBP, B-ACBP
and PBR play a role in the pathogenesis of oesophageal
tumours and possibly in carcinogenic angiogenesis.
publication.page.subject
Citation
Histology and Histopathology, Vol.32, nº6, (2017)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/