Publication: A salvianolic acid 8-rich fraction
of Sa/via miltiorrhiza induces neointimal
cell apoptosis in rabbit angioplasty model
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Date
2001
Authors
Hung, H.-H. ; Chen, Y.-L. ; Lin, S.-J. ; Yang, S.-P. ; Shih, C.-C. ; Shiao, M.-S. ; Chang, C.-H.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Apoptosis has been suggested to participate
in stabilizing cell number in restenosis. Salvia
miltiorrhiza (SM) Bunge which is a Chinese herb widely
used for the treatment of cardiovascular disorders
contains a potent antioxidant, Salvianolic ac id B. To
determ ine whether the antioxidant affects vascular
apoptosis, the present study examined the frequency of
apoptotic cell death in atherosclerotic plaques and in
restenotic lesions of cholesterol-fed rabbits. New
Zealand White rabbits were treated with a normal diet
(normal), a 2% cholesterol diet (HC), a 2% cholesterol
diet and endothe lial denudation (HC-ED), a 2 %
cholesterol diet with 5% water-soluble extract of SM
(4.8 glKg B. W./day) and endothelial denudation (HCED-SM), or with a 2% cholesterol diet containing
probucol (0.6 glkg B.W./day) and endothelial denudation
(HC-ED-probucol). Apoptosis and associated cell types
were examined in serial paraffin sections by in situ
terminal deoxynucleotidyl transferase-mediated dUTP
nick end labeling and immunohistochemistry. The
expression of p53, an apoptosis-related protein, was also
examined. Apoptosis was mainly detected in the
neointima of the three gro ups with endothe lial
denudation. The percentage of apoptotic cells in SMtreated group (68.5±5.9%) was significantly higher than
that of normal (0 %), HC (1.9±1.2 %), HC-ED
(46.1±5.4%), and probucol-treated (32.8±3.9%) groups.
The SM treatment markedly reduced the thickness of the
neointima which was mainly composed of smooth
muscle cells with few macrophages. In accordance with
the apoptotic cell counts, positive immunoreactivity for
p53 was observed in restenotic lesions from HC-ED,
SM-treated and probucol-treated groups but not in the
intima of the other two groups. These results suggest that
the treatment with salvianolic acid B-rich fraction of SM
induces apoptosis in neointima which in turn may help prevent the neointimal thickening.
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