Publication: Heat shock proteins and survivin, Relationship and effects on proliferation index of retinoblastoma cells
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Date
2008
Authors
Jiang, L.-B. ; Liu, X.-Q. ; Li, B. ; He, X.-J. ; Jin, Y.L. ; Li, L.-Q. ; Gao, F. ; Wang, N.-L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Survivin and HSPs (heat shock proteins) are
important anti-apoptotic proteins. However, limited
research has been done regarding the collective effects
of HSPs and survivin on the proliferative activities of
RB cells. The purpose of this study was to narrow this
gap by focusing on the expression of HSP70 and HSP90
and the interaction of these proteins with survivin. The
proliferative activities of RB cells were analyzed by
assessing the Ki-67 labeling index. Ki-67 recognizes a
nuclear antigen expressed in all phases of the cell cycle
except G(0) and early G(1), which makes it an excellent
marker of cells in the proliferative phase.
Immunohistochemical procedures were performed on
retinal tissues from 43 RB patients who had undergone
enucleation. Expression of HSP70, HSP90 and survivin
was found in 65.12%, 86.05% and 62.79% of the cases
respectively. No expression of any of these markers was
found in normal retinal tissues. Expression of survivin
was more frequent when HSP90 was detected than when
HSP90 was not detected (P<0.05). The Ki-67 labeling
index was higher in cases in which HSP90 or survivin
was found than in cases in which neither protein was found (P<0.05). The Ki-67 labeling index was higher in
cases positive for both HSP90 and survivin than in cases
in which neither protein or only one protein was found
(P<0.05). Expression of HSP70 neither correlated with
that of survivin, nor had any significant effect on the Ki-
67 labeling index (P>0.05). Although expression of
HSPs and survivin and the Ki-67 labeling index did not
correlate with histopathologic typing of RB (P>0.05),
our findings demonstrate that expression of HSP90
correlates with that of survivin in RB and the coexistence
of survivin and HSP90 probably plays an
important role in cellular proliferation in RB. Further
work is indicated to clarify the role of these processes in progression of RB.
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