Publication: Rat hair follicle-constituting cells labeled by a newly-developed somatic stem cell-recognizing antibody: a possible marker of hair follicle development
Authors
Ichikawa, Chisa ; Izawa, Takeshi ; Juniantito, Vetnizah ; Tanaka, Miyuu ; Hori, Mayuka ; Tanaka, Katsuhiro ; Takenaka, Shigeo ; Kuwamura, Mitsuru ; Yamate, Jyoji
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
. A3 was generated as an antibody recognizing
somatic stem cells in rat tissues. We investigated the
distribution of A3-positive cells in developing rat hair
follicles by immunolabeling. A3-positive cells began to
be seen in the hair germ and peg in fetuses and neonates;
the positive cells were epithelial cells above basal cells.
Furthermore, A3-positive cells were seen in the outer
root sheath adjacent to the bulge in mature hair follicles.
Double immunofluorescence revealed that these A3-
positive epithelial cells reacted to E-cadherin (for all
epithelial elements) but not to CK15 (for basal
cells/epithelial stem cells) or to nestin (for stem cells),
indicating that A3-positive epithelial cells are suprabasal
cells in the developing epidermic hair follicle.
Additionally, spindle-shaped mesenchymal cells
surrounding the hair peg and mature hair follicle reacted
to A3; in double immunofluorescence, the A3-positive
cells were located outside collagen type IV-positive
glassy membrane, and reacted to vimentin (for
mesenchmal cells), Thy-1 (for immature mesenchymal
cells), CD34 (for stem cells) and nestin, but not to α-
smooth muscle actin (for myofibroblasts); the positive
cells were regarded as immature mesenchymal cells with
stem cell nature in the connective tissue sheath of
developing hair follicles. A3-positive epithelial and
mesenchymal cells did not show proliferating activity.
Collectively, it is considered that A3-positive cells seen
in developing rat hair follicles may be quiescent postprogenitor
cells with the potential to differentiate into
either highly-differentiated epithelial or mesenchymal
cells. A3 would become a useful antibody to know the
kinetics of rat hair follicle-constituting cells.
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