Publication: Scanning electron microscopic examinations on retarded bone defect healing in
spontaneously diabetic BB/O(ttawa)K(arlsburg) rats
Authors
Follak, N. ; Klöting, I. ; Ganzer, D. ; Merk, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To date, no detailed knowledge from animal
experiments is available on the kind and extent of
osseous and mineral metabolic disorders in genetically
determined, insulin-dependent Type I diabetes. The
purpose of this study was to examine the influence of the
diabetic metabolic state in spontaneously diabetic
BB/O(ttawa)K(arlsburg) rats on bone defect healing.
Eighty spontaneously-diabetic BB/OK rats with a
blood-glucose value of 391±106 mg% (mean ± SD) at
the time of manifestation were used in the study. Based
on blood-glucose values at the time of surgery (mg%),
postoperative blood-glucose course (mg%) and
postoperative insulin requirements (IU/kg), the animals
were divided into groups with well-compensated (n=40,
170±101 mg%; 221±120 mg%; 2.1±1 IU/kg) or poorly
compensated (n=40; 371±158 mg%; 357±83 mg%;
5.2±1.4 IU/kg) metabolic state. Forty LEW.1A rats
served as the normoglycemic controls (95±18 mg%).
Using a 1-mm-diameter Kirschner wire, a hole of
femoral bone ca. 1 cm proximal to the knee joint space
was centrally drilled. Ten animals from each group were
killed on postoperative days 7, 14, 24, and 42, and
specimens were taken for analysis. Using SEM to
measure regions of new bone semiautomatically and
quantitatively, also determining the number, area, and
circumference of regions not yet filled with new bone. Up to postoperative day 14, very significant
differences (p<0.0001) for all investigated characteristics
were found between the spontaneously-diabetic BB/OK
rats and the control animals – in favor of the controls –
and up to postoperative day 24 within the group of
spontaneously-diabetic BB/OK rats, where the wellcompensated
animals had significantly better results in
terms of number and area of regions of bone not yet
filled with new bone formations. Forty-two days
postoperatively, SEM observations showed no differences between examination groups.
The process of bone defect healing in
spontaneously-diabetic rats was disturbed only in the
early phase and exhibited retardation in its progression.
After 42 days, bone defect healing was complete,
regardless of the diabetic metabolic state; no differences
were detected with the SEM between examination
groups at this time point.
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