Publication: Heat-induced antigen retrieval of epoxy sections for electron microscopy
Loading...
Date
2001
Authors
Brorson, S.H.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The purpose of this manuscript is to review
the literature on the use of heat-induced antigen retrieval
methods to enhance the immunolabeling of epoxy
sections at the electron microscopical level. The history
of the development of antigen retrieval by heating
formaldehyde fixed paraffin sections in a buffer solution
is given in short, and how this technique has been
extended to resin sections and in particular epoxy
sections is explained. Theories for the mechanism of
enhancement of the immunolabeling of epoxy sections
by the heat-retrieval method are discussed, and it is
finally speculated whether most of the mechanisms for
antigen retrieval on epoxy sections in heated buffer
solution are essentially the same as for conventional
immunoenhancing by deplastizing and etching. The
more accelerator used in the processing of the tissue the
more intense the immunolabeling of the heated epoxy
sections becomes. The intensity of immunolabeling of
the epoxy sections increases with the temperature in the
heated buffer solution, and the intensity is significantly
higher at high autoclave temperatures than at 95 OC,
Heat-induced antigen retrieval is also compared with
other, conventional techniques for enhancing the
immunolabeling of epoxy sections.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.