Publication: Actin cytoskeletal organization in human osteoblasts
grown on different dental titanium implant surfaces
Loading...
Date
2007
Authors
Salido, M. ; Vilches, J.I. ; Gutiérrez, J.L. ; Vilches, J.
item.page.secondaryauthor
item.page.director
Publisher
Murcia: F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The understanding of the cellular basis of
osteoblastic cell-biomaterial interaction is crucial to the
analysis of the mechanism of osseointegration. Cell
adhesion is a complex process that is dependent on the
cell types and on the surface microtopography and
chemistry of the substrate.
We have studied the role of microtopography in
modulating cell adhesion, in vitro, using a human
osteoblastic cell line for the assessment of actin
cytoskeletal organization. Through application of CLSM
combining reflection and fluorescence, 2D or 3D images
of cytoskeleton were obtained.
On smooth surfaces, Ti CP machined, predominantly
planar bone cells with an axial ratio of 1.1 were
randomly oriented, with stress fibers running in all
directions, and thin filopodia.
On TiCP Osseotite® surfaces the osteoblastic cells
conformed to the irregular terrain of the sustrate with
focal adhesion sites only established on the relative
topographical peaks separated for a longer distance than
in the machined surface, and defined wide lamellopodia
and long filopodia, with enhanced expression of stress
fibers, forming large clear focal contacts with the rough
surface. The cytoskeletal organization of cells cultured
on rough titanium supports an active role for the
biomaterial surface in the events that govern osteoblastic
cell adhesion. The results enforce the role of the rough
sustrate surface in affecting osteoblastic cell adhesion
and provide valuable information for the design of
material surfaces that are required for the development
of an appropriate osteogenic surface for osteoblastic
anchorage, compared to machined surface, in dental
implants.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.