Publication: Irradiation of the rabbit cornea with UVB rays stimulates the expression of
nitric oxide synthases-generated nitric oxide and
the formation of cytotoxic nitrogen-related oxidants
Authors
Cejkova, J. ; Ardan, T. ; Cejka, C. ; Kovaceva, J. ; Zídek, Z.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Until now, the role of nitric oxide (NO) in
cornea irradiated with UVB rays remains unknown.
Therefore, we investigated nitric oxide synthase isomers
(NOS), enzymes that generate NO, nitrotyrosine (NT), a
cytotoxic byproduct of NO, and malondialdehyde
(MDA), a byproduct of lipid peroxidation, in rabbit
corneas repeatedly irradiated with UVB rays (312 nm,
1x daily for 6 days, the dose per day 1.01 J/cm2) using
immunohistochemical methods. The biochemical
measurement of nitrite and nitrate has been used for the
indirect investigation of NO concentration in the
aqueous humor. Results show that in contrast to normal
corneas, where of the NOS isomers only endothelial
nitric oxide synthase (NOS3) was expressed in a
significant amount (in the epithelium and endothelium),
in irradiated corneas all NOS isomers (also brain nitric
oxide synthase, NOS1, and inducible nitric oxide
synthase, NOS2) as well as an indirect measure of
ONOO-formation and MDA were gradually expressed,
first in the epithelium, the endothelium and the
keratocytes beneath the epithelium and finally in the
cells of all corneal layers and the inflammatory cells that
invaded the corneal stroma. This was accompanied by an
elevated concentration of NO in the aqueous humor. In
conclusion, repeated irradiation with UVB rays evoked the stimulation of NO production, peroxynitrite
formation (demonstrated by NT residues) and lipid
peroxidation (evaluated by MDA staining).
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