Publication:
Tracing the accumulation and effects of mercury uptake in the previtellogenic ovary of crucian carp Carassius auratus gibelio by autometallography and caspase-3 immunohistochemistry

Simple item page
dc.contributor.authorZarnescu, Otiliaes
dc.date.accessioned2013-09-25T08:07:04Z
dc.date.available2013-09-25T08:07:04Z
dc.date.issued2009
dc.description.abstractThe aims of the present study were to apply the AMG technique for localization of mercury at the light and electron microscopic level in the ovary of crucian carp after exposure to mercuric chloride and to find out if this heavy metal induces expression of caspase-3. Depending on the stage of ovarian follicle development, two patterns of mercury accumulation have been found in previtellogenic ovary of crucian carp. The first mercury accumulation pattern has been found in the early previtellogenic oocyte without zona radiata. In these oocytes, mercury accumulates into an ooplasmic region that seems to correspond to the Balbiani body (32-65 μm oocyte diameter), throughout the cytoplasm (84-116 μm oocyte diameter) and in the cortical cytoplasm (~180 μm oocyte diameter). The second mercury accumulation pattern has been found in the late previtellogenic oocyte with cortical alveoli (229- 330 μm oocyte diameter). Ultrastructural observations have shown grains of silver-enhanced mercury inside coated vesicles, the cortical lysosome-like bodies or multivesicular bodies and cortical alveoli. Immunohistochemistry reaction for caspase-3 was positive in nuclei of the early previtellogenic oocyte and Balbiani body.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/36084
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAutometallographyes
dc.subjectUltrastructurees
dc.subject.other57 - Biologíaes
dc.titleTracing the accumulation and effects of mercury uptake in the previtellogenic ovary of crucian carp Carassius auratus gibelio by autometallography and caspase-3 immunohistochemistryes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Tracing the accumulation and effects of mercury uptake in the previtellogenic ovary of crucian carp.pdf
Size:
4.71 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.24, nº2 (2009)