Publication: Remodelling of collagen fibres in the placentas of women with venous insufficiency during pregnancy
Authors
Ortega, Miguel A. ; Asúnsolo, Ángel ; Álvarez Rocha, María J. ; Romero, Beatriz ; De León-Luis, Juan ; Álvarez Mon, Melchor ; Buján, Julia ; García Honduvilla, Natalio
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-948
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info:eu-repo/semantics/article
Description
Abstract
Haemodynamic changes produced during
pregnancy lead to elevated venous pressure in the legs
and an increased resting consumption of oxygen. These
events can cause varicose veins, or venous insufficiency
(VI), which by creating an environment of hypoxia
could affect the structure and function of the placental
barrier. This study assesses the remodelling state of the
placental villi by examining differences in collagens
with a known role in villus structure and in placental
barrier permeability between patients with and without
VI.
Samples of 67 placentas from women with VI
(n=24) and without VI (n=43) during their pregnancy
were processed for gene and protein expression analysis
of COL-I, COL-III, MMP-2 and MMP-9 by RT-qPCR
and immunohistochemistry.
While no differences in COL-I expression levels
were detected in the samples from women with and
without VI, significant differences did emerge in both
gene and protein expression levels of COL-III.
Importantly, COL-I/III ratios were reduced in the VI
group compared to controls. MMP-2 activity was similar
in the two groups while MMP-9 levels were significantly
elevated in VI with greatest expression differences
observed at the level of the decidual cells.
Mothers who developed VI during pregnancy
showed significantly higher COL-III and MMP-9 levels
consistent with a state of remodelling of the placental
villi.
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Citation
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