Publication: Skeletal muscle regeneration and Trypanosoma cruzi-induced myositis in rats
Authors
Maldonado, I.R.S.C. ; Ferreira, M.L. ; Camargos, E.R.S. ; Chiari, E. ; Machado, C.R.S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Although Chagas’ disease is known to
provoke severe acute myositis, information on muscle
regeneration is missing. The current paper shows that
during T. cruzi infection in rats, skeletal muscle
parasitism and the consequent inflammatory process are
higher in muscle with a high proportion of type-I
myofibres (soleus and diaphragm). Immunohistochemistry
showed an acute inflammatory process
characterized by ED1+ and ED2+ macrophages, CD8+
lymphocytes, and NK cells. Parasite-nest rupture
provoked segmental degeneration of myofibres followed
by regeneration. These phenomena were observed at
both light and transmission electron microscopy levels.
Myofibre regeneration involved activation of satellite
cells assessed by the expression of MyoD, a musclespecific
transcription factor. Ultrastructural evidence of
fusion of myoblast-like cells with the intact segment of
degenerating fibres has been provided. At the chronic
phase no signs of fibrosis were found, but sparse and
small inflammatory foci were found. Our results argue
against the relevant participation of autoimmunity
phenomena in both acute and chronic phases and furnish
a new view for explaining histopathological findings in
human patient muscles.
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