Publication: Importance of interfacial water in permeabilization of ceramide bilayers
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Date
1998
Authors
Neitchev, V. ; Kostova, E. ; Dimitrov, A.S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The role of structured water at the membrane-
aqueous interface in regulating the water
permeability functions of the epidermal horny layer
(Stratum corneum) is not clear at present. The subject of
this review is the effect of perturbed interfacial water
structure on the relative water permeability properties in
shrinkage experiments of reconstituted vesicle
suspensions of ceramides and cholesterol in the gel
phase. Water structure was perturbed by a series of
reagents known to affect the phase equilibrium of lipid
assemblies. Multilamellar bilayers containing synthetic
ceramides N-palmitoyl-D-L-dihydrosphingosine, and Noleoyl-
D-sphingosine were used as model membranes.
The reagent binding to the ceramide membrane was
determined by fluorescence measurements with 1-
anilino, 8-naphtalene sulphonate (ANS). A dependence
of number of binding sites for ANS on the type of
reagent was found. The change of relative water
permeability with concentration of reagents was studied.
Analysis of these data reveals that the anomalous
behaviour of relative water permeability with the
concentration of reagents used is due to concentrationdependent
structural transitions of the bound water at the
membrane-aqueous interface. Different possibilities of
interaction of the reagents with the interface are
discussed. At the end, it is suggested that the interfacial
water in multilamellar ceramide structures may also
contribute to the water holding and permeability barrier functions of cell membrane complex within the stratum
corneum.
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