Publication: Detection of PR-39, a porcine host defence peptide, in different cell sub-linages in pigs infected with Actinobacillus pleuropneumoniae
Authors
Gabner, S. ; Egerbacher, M. ; Gasse, H. ; Hewicker Trautwein, M. ; Höltig, D. ; Waldmann, K. H. ; Blecha, F. ; Saalmüller, A. ; Hennig Pauka, I.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-869
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info:eu-repo/semantics/article
Description
Abstract
Innate immunity is critically important for
the outcome of infection in many diseases. It was
previously shown that cathelicidin PR-39, an important
porcine multifunctional host defence peptide, is elevated
in bronchoalveolar lavage fluid and respiratory tract
tissue after experimental infection with Actinobacillus
pleuropneumoniae (A.pp.). To date, neutrophil
polymorphonuclear leukocytes (PMNs) are thought to be
the only source of PR-39.
The aim of this study was to further characterize PR39+ cells and selected immune cell populations in lung
tissue during the peracute (7-10 hours), acute (2 days),
reconvalescent (7 days) and chronic (21 days) stages of
experimental infection with A.pp. serotype 2. In total, six
mock-infected control pigs and 12 infected pigs were
examined. Using immunofluorescence double-labeling,
antibodies against PR-39 were combined with antibodies
against CD3 (T-cells), CD79 (B-cells), Iba1 (activated
macrophages), TTF-1 (lung epithelial cells expressing
surfactant proteins), macrophage/L1 protein and
myeloperoxidase (MPO, cells of the myeloid linage).
In the peracute and acute phases of infection, total
PR-39+ cells and myeloid linage cells increased, whereas
CD3+ cells and TTF-1+ cells decreased. Double labeling
revealed that most Macrophage/L1 protein+ cells and to
a lesser extent MPO+ cells co-expressed PR-39. In
addition, few bronchial epithelial cells and type 2
alveolar epithelial cells (both identified with TTF-1)
produced PR-39. Occasionally, CD3+ T cells expressing
PR-39 were seen in infected animals.
Taken together, this study identifies cell types, other
than PMNs, in lungs of A.pp.-infected pigs that are
capable of producing PR-39. In addition, these findings
provide further insights into the dynamics of different
immune cell populations during A.pp.-infection.
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Citation
Histology and Histopathology, Vol.32, nº10, (2017)
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