Publication: An autoradiographic study of the uptake of tritiated proline by osteoblasts during hibernation
Loading...
Date
1986
Authors
Steinberg, B. ; Singh, I.J. ; Mitchell, O.G.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Twenty-four LSH and LVG strain golden
hamsters, Mesocricetus auratus, were used. Experimental
animals were maintained at 5 C and allowed to hibernate.
Control animals were kept at 27 C. Six animals
(3 experimental, 3 control) were injected subcutaneously
with I pCi of 'H-proline/gm body wt. (Spec. act.
3 Ci/mM) after hibernation lasting 12 hours, I day, 3 days,
or 7 days. Animals were killed I hour after injection
and autoradiographs were prepared from 5 Frn thick
ilecalcified sections of femurs.
A greater number of endosteal cells were labeled than
periosteal cells and also exhibited a greater magnitude
of labeling throughout the study. Differences between
endosteal and periosteal cells both in percentage of
cells labeled and magnitude of labeling were maximum
in control animals and progressively decreased with
increasing periods of hibernation. A reduction in synthesis
of matrix proteins during the early period of hibernation
was seen and was attributed to a significant reduction both
in average cell activity and in the number of active cells
during hibernation. The latter phenomenon apparently
made a large contribution to the reduced matrical synthesis.
'H-proline uptake by osteoblasts probably retlects
the reduced requirements of matrical synthesis during hibernation.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.