Publication: Reg I protein in healthy and seminoma human testis
Authors
Mauro, V. ; Carette, D. ; Chevallier, D. ; Michiels, J.F. ; Segretain, D. ; Pointis, G. ; Sénégas-Balas, F.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Regenerating gene (Reg), encodes a
secretory protein with growth and differentiation
stimulating effects mostly in digestive tissues.
Overexpression of Reg proteins and specifically of Reg
I, one member of the Reg family, is associated with
several human diseases and cancers. In the present study
we analyzed the expression of Reg I in normal rodent
and human testes where germ cells normally proliferate
and differentiate into spermatozoa, and in seminoma
testis, the most common cancer of young men. Western
blot analyses demonstrated the presence of a specific
band at 19 kDa in human and rodent testis extracts.
Immunofluorescence and deconvolution microscopy
demonstrated that Reg I was present within the
seminiferous tubules in both Sertoli and germ cells. By
using a Sertoli cell line we demonstrated that Reg I was
localized at the plasma membrane even in the absence of
contact between neighboring cells and appeared before
the tight junction associated protein ZO-1 was revealed
at this location. Reg I was strongly expressed in human
seminoma testis tissue and in a human tumor germ cell
line where the immunoreactive signal was mainly
detected at the plasma membrane level. These data
showing for the first time the weak presence of Reg I in
the normal testis and its strong expression in the testis
cancer suggest a potential role of Reg I in normal and
neoplastic germ cell proliferation.
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