Publication: lmmunohistochemical detection of metallothionein in carcinomatous and normal human gastric mucosa
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Date
2000
Authors
Tuccari, G. ; Giuffre, G. ; Arena, F. ; Barresi, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Utilising a specific monoclonal mouse
antibody (E9), metallothionein (MT) expression has
been immunohistochemically investigated in 112
formalin-fixed paraffin-embedded surgical gastric
samples, 38 of which were early carcinomas (EGC) and
74 advanced ones (AGC); clinico-pathological details
and follow-up data (ranging from 3 to 197 months, mean
60.5 months) were available. Eighty-nine portions of
gastric mucosa adjacent to examined carcinomas
(transitional mucosa) were also analysed; in addition, 22
biopsies of normal gastric mucosa were studied as tissue
control. The MT immunoreactivity was evaluated by
staining and intensity-distribution scores. A various MT
positivity was appreciable in the cytoplasm and nucleus
of antrum or body gastric epithelia1 cells in 100% of
normal control biopsies. 751112 (67%) gastric
carcinomas showed MT immunoreactivity with a
significant lower expression in AGC. No relationships
were encountered between MT immunostaining and
clinico-pathological data; in addition, no difference in
the Kaplan-Meier survival curves of patients with
various MT expression was achieved. When the
transitional mucosa was examined, 84/89 (94%) samples
were stained although the immunoreaction was not
always concordant with that encountered in adjacent
carcinomatous elements. The significant statistical
decrease of MT scores observed by us moving from
normal to neoplastic gastric mucosa allows us to exclude
the hypothesis of an overexpression of MT in gastric
carcinomas.
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