Publication: Blood-borne cells involved in arterial repair upon experimental incision injury
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Date
2008
Authors
Pieri, L. ; Rinaldi, B ; Domenici Lombardo, L. ; Bacci, S. ; Filippelli, A. ; Capuano, A. ; Rossi, F. ; Romagnoli, P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Summary. We had previously shown that
microscopically detectable infiltration of dendritic cells
and expression of Hsp47 in tissue lysates occur during
repair upon experimental arterial injury. We have further
analysed here the cell types involved in the repair
process by histology, electron microscopy and
immunofluorescence. Rat carotid arteries were subjected
to brief crushing and full thickness incision and were
analysed up to 21 d thereafter. Adhesion and activation
of platelets occurred 3 h after surgery. A neointima had
formed 7 d after surgery, where immature cells entered
from the lumen and gave rise to cells rich in organelles
of the secretory pathway and endowed with bundles of
phalloidin-binding microfilaments. Alpha smooth
muscle-positive, secretory and contractile smooth
muscle cells were found in the neointima 14 and 21 d
after injury. Seven to 21 d after surgery, endothelial cells
appeared immature and the newly formed tissue
contained MHC-II positive, CD43 positive dendritic
cells which clustered with lymphocytes, a few
macrophages containing apoptotic remnants and cells
labelled for Hsp47. Thin elastic fibrils appeared in the
neointima 21 d after injury. The results suggest that the
response to acute arterial incision injury is mediated by
blood borne cells which differentiate along multiple
pathways; the process evolves without reaching
stabilization within the observed time lapse; the
secretion of extracellular matrix is marked by the
expression of Hsp47; and the constant presence of
dendritic cells clustered with lymphocytes makes these
cells candidate to a pivotal role in the tissue response to
injury.
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