Publication: Mitochondrial bioenergetics and distribution in living human osteoblasts grown on implant surfaces
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Date
2009
Authors
Salido, Mercedes ; Vilches Pérez J.Ignacio ; González, Juan L. ; Vilches, José
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Osseointegration of implants is crucial for
the long-term success of oral implants. The periimplant
bone formation by osteoblasts is strongly dependent on
the local mechanical environment in the interface zone.
Robust demands for energy are placed on osteoblasts
during the adhesion process to solid surfaces, and
mitochondria are capital organelles in the production of
most of the ATP needed for the process. We have
assessed the relationship between osteoblast
differentiation and mitochondrial bioenergetics in living
cells grown on two different titanium surfaces, in order
to provide valuable information for the design of
material surfaces required for the development of the
most appropriate osteogenic surface for osteoblastic
anchorage. Combined backscattered and fluorescence
confocal microscopy showed that in flat cells grown on a
machined surface, highly energized mitochondria were
distributed along the cell body. In contrast, cells grown
on the rough surface emitted long protrusions in search
of surface roughness, with actin stress fibers clearly
polarized and highly energized mitochondria clustered at
focal adhesion sites. This report using normal human
osteoblastic cells indicates that these cells are especially
sensitive to surface cues through energy production that
enhances the necessary adhesion required for a
successful osseointegration.
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