Publication: Cholinergic, nitrergic and peptidergic (Substance P- and
CGRP-utilizing) innervation of the horse intestine. A
histochemical and immunohistochemical study
Authors
Domeneghini, C. ; Radaelli, G. ; Arrighi, S. ; Bosi, G. ; Dolera, M.
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Publisher
Murcia : F. Hernández
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Description
Abstract
The small and large intestine of adult horses
were histochemically and immunohistochemically
investigated in order to evidence components of the
intramural nervous system. The general structural
organization of the intramural nervous system was
examined by using Nissl-thionin staining as well as the
anti-neurofilament 200 (NF200) immunoreaction, which
demonstrated the presence of neurons in the submucous
as well as myenteric plexuses. The additional presence
of subserosal ganglia was shown in the large intestine.
Acetylcholinesterase (AChEase) activity was observed
in both the submucous and myenteric plexuses.
Localization of acetylcholine-utilizing neurons was also
evidenced by immunohistochemical reactions for
choline acetyltransferase (ChAT) and vesicular
acetylcholine transporter (VAChT). With both
histochemistry and immunohistochemistry possible
cholinergic nerve fibres were detected in the inner
musculature. The two possible cholinergic co-mediators
Calcitonin Gene-Related Peptide (CGRP) and Substance
P (SP) have been investigated by an immunohistochemical
approach. CGRP immunoreactivity was
detected in roundish nerve cell bodies as well as in nerve
fibres of the submucous plexus, whereas SP
immunoreactivity was evidenced in nerve fibres of the
tunica mucosa, in nerve cell bodies and fibres of the
submucous plexus and in nerve fibres of the myenteric
plexus. NADPH-diaphorase reactivity, which is linked to
the synthesis and release of nitric oxide, was detected in
nerve cell bodies and nerve fibres of both the submucous
and myenteric plexuses as well as in a subserosal
localization of the large intestine. The nitrergic
components were confirmed by the anti-NOS (nitric
oxide synthase) immunoreaction. Results are compared
with those of other mammals and related to the complex intestinal horse physiology and pathophysiology.
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