Publication: Mineralization of human premolar occlusal fissures. A quantitative histochemical microanalysis
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Date
2000
Authors
Campos, Antonio ; Rodriguez, I.A. ; Sanchez-Quevedo, M.C. ; García, J.M. ; Nieto-Albano, O.H. ; Gómez de Ferraris, M.E.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The mechanisms of cariogenesis in occlusal
fissures remain elusive because of limited information
about fissure structure and wall mineralization. The
purpose of the present study was to determine the
correlation between morphological patterns in occlusal
fissures in human premolars and quantitative
histochemical patterns of mineralization in the walls of
these formations. We used scanning electron microscopy
and quantitative X-ray microanalysis with the peak-tolocal
background ratio method and microcrystalline
calcium salts as standards. We distinguished three
morphological patterns of fissures in scanning electron
microscopic images. The wall of the fissures was less
mineralized than the control enamel in all three types of
fissures. Because the fissure walls are hypomineralized,
we suggest that practicing dentists should take into
account the degree of mineralization when they are
preparing the fissures for the application of sealant.
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