Publication: A comparison between double and triple therapies of octreotide,
galanin and serotonin on a rat colon carcinoma
Authors
Sitohy, B. ; El-Salhy, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Sixty female nude mice (C578L/6jBom-nu)
were injected with 100µl cell suspension containing
2x106 viable cells of an N-methyl-N-nitroguanidineinduced
rat colonic adenocarcinoma. After seven days
the animals were divided into five groups. The first
group received only saline and served as a control group.
The second group received a triple therapy of octreotide,
galanin and serotonin (20 µg/kg). The last three groups
received double therapies of octreotide/galanin,
octreotide/serotonin or galanin/serotonin (20 µg/kg).
They were treated twice a day for five days. Tumour
volume and weight, relative volume density of tumourfeeding
blood vessels and of tumour necrotic tissue, as
well as apoptotic and proliferation indices were
determined. Animal weight, food consumption, faeces
weight and its water content were recorded before and
after treatment. Tumour volume was significantly
reduced only in the group that received the triple
therapy. The volume density of the tumour-feeding
blood vessels was significantly reduced in the treated
groups with the exception of the group that received
octreotide and serotonin. Increased relative volume
density of tumour necrotic tissue occurred only in the
group treated with triple therapy. Apoptotic indices were
significantly increased in all treated groups. No
statistical difference was found between treated animals
and controls regarding proliferation indices, food consumption, faeces weight and water content or animal
weight. In conclusion, double therapy using two of the
gastrointestinal bioactive substances, octreotide, galanin
and serotonin, has certain effects on colon cancer cells.
To cause a considerable tumour necrosis, triple therapy
seems to be required. Both double and triple therapy
seem to lack obvious side-effects.
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